Banyaknya masyarakat yang memperoleh perawatan pada beberapa instansi layanan kesehatan menjadikan adanya alasan mengenai pentingnya kegiatan pengelolaan informasi kesehatan pasien yang terintegrasi. Hal ini berfungsi untuk menyediakan informasi kesehatan secara komprehensif kepada pasien dan staf medis dalam membantu pelayanan kesehatan yang lebih baik. Artikel ini mengkaji elemen-elemen yang diperlukan dan model dalam kegiatan Health Information Management yang terintegrasi. Kajian artikel ini dilakukan secara deskriptif dengan mengkaji 23 literatur yang terbit dari tahun 2007-2017, yang diperoleh dari jurnal-jurnal yang dilanggan perpustakaan UGM, Google Scholar dan PubMed Health, mengenai pengelolaan informasi kesehatan pasien. Hasil penelitian ini menjelaskan bahwa elemen yang diperlukan dalam penerapan Health Information Management yang terintegrasi dapat dibagi ke dalam lima elemen, yaitu: 1. Sumber Daya Manusia, 2. Kebijakan dan kelembagaan, 3. Penerapan sistem, 4. Data dan pengelolaannya, 5. Akses dan integrasi data. Pada model pengelolaan informasi kesehatan pasien yang terintegrasi, harus menyediakan akses terhadap informasi kesehatan pasien dan memungkinkan pertukaran data yang dikelola beberapa petugas medis, pengelola data pasien dan rumah sakit lain. Hal ini didukung oleh sebuah sistem informasi kesehatan yang dilengkapi dengan sistem keamanan data dan adanya dukungan dari kepala rumah sakit.Kata kunci: Pengelolaan informasi kesehatan; layanan kesehatan di rumah sakit; integrasi informasi kesehatan pasien

Euphorbia heterophylla. Desf (kate mas) menunjukkan aktivitas penurunan glukosa darah, serta memiliki sifat antioksidan dan antiinflamasi. Penelitian ini bertujuan untuk mengeksplorasi potensi ekstrak etanol daun Euphorbia heterophylla Desf (EEH) sebagai anti resistensi insulin dalam pengelolaan diabetes mellitus tipe 2 (DMT2). Tujuan penelitian ini untuk mengetahui efek dan dosis efektif Ekstrak Etanol Daun Kate Mas (Euphorbia heterophylla Desf), disingkat EEH, terhadap tikus yang diinduksi makanan tinggi karbohidrat, lemak, dan fruktosa 30%. Metode penelitian dilakukan secara in vivo menggunakan 24 tikus jantan putih galur wistar yang dibagi menjadi 6 kelompok, yaitu kelompok kontrol normal, kontrol negatif, kontrol positif, EEH 50 mg/KgBB, 100 mg/KgBB, dan 200 mg/Kg BB dengan melakukan tes  kadar glukosa darah (KGD), pengukuran konstanta tes toleransi insulin (KTTI) dan histopatologi sel pankreas. Hasil penelitian menunjukkan bahwa EEH pada semua kelompok dapat menurunkan KGD (92,25 mg/dL±7,04 untuk 50 mg/KgBB; 99,75 mg/dL±13,57 untuk 100 mg/Kg BB; 117,00 mg/dL ±0,85 untuk 200 mg/Kg BB; dan 89,00 mg/dL ±5,34 untuk metformin) dibandingkan dengan kelompok kontrol negatif (132,00 mg/dL ±7,16). EEH 50 mg/Kg BB secara signifikan dapat meningkatkan sensitivitas insulin (3,95±0,53) dibandingkan dengan kelompok negatif (1,24±0,98). Hasil histologi pankreas menunjukan EEH pada semua kelompok uji dapat menurunkan jumlah nekrosis sel pankreas dibandingkan dengan kelompok kontrol negatif.  Kesimpulannya, EEH memiliki potensi untuk dieksplorasi lebih lanjut untuk aktivitas dalam meningkatkan sensitivitas insulin.

Bitter leaf, Vernonia amygdalina (VA), is used in various alternative medicine in several countries,including Indonesia. This study aims to evaluate the antioxidant activity of VA leaves by three differentin vitro methods. The antioxidant activity was evaluated by radical scavenging of 1,1-diphenyl-2-picryl-hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and cupric reducing antioxidantcapacity (CUPRAC). The antioxidant capacity by FRAP and CUPRAC methods were expressed asmg of quercetin equivalents (QE) per g extract (mgQE/g) and mg of ascorbic acid equivalents (AAE)per g extract (mgAAE/g), respectively. All methods were measured by UV-Visible spectrophotometryat maximum wavelength. The results exhibited that VA leaves ethanolic extract contains flavonoidphenolic.The VA leaves ethanolic extract has an antioxidant activity by scavenging the DPPH methodwith the half inhibition concentration (IC50) was 8,22 μg/mL. The antioxidant capacity of VA leavesethanolic extract by FRAP and CUPRAC methods was 21,03 mgQE/g and 160,64 mgAAE/g extract,respectively. The VA leaves ethanolic extract has a powerful antioxidant potency which might correlateto the flavonoid-phenolic content in the extract.

The quality control of herbal medicine should be started from the determination of the active substance at harvest time. The Raman spectrometry has been used for this propose. The aim of this study is to determine the quantification of curcumin in turmeric rhizome (Curcuma longa Linn.) using Raman spectroscopy combined with multivariate analysis of PLS-R that are expected to provide reference method for quality control in turmeric rhizome, especially for raw materials of Herbal drugs. Parameters that can be used for analysis of curcumin levels on turmeric rhizome obtained using intensity of data Raman and the data obtained from the standard method will be processed with multivariate analysis methods PLS-R. The validation value of quantification result using Raman-PLSR is seen from R2 value of 0.957, RMSEC value of 0,199 and p-value of 0.00. The study showed the developed method could be implemented on to determine the prediction quantification of raw material herbal medicine.Keyword : Curcumin,  PLS-R, Turmeric, Raman Spectroscopy

The inappropriate use of antibiotics can lead to pathogenic bacteria developing multiple drug resistance, making infectious disease treatment crucial. Thus, the discovery of novel antibiotics from natural materials, such as plant extracts or their combinations, is critically needed. Extracts of green betel (Piper betle) and Moringa (Moringa oleifera) leaves are known to have antimicrobial activity. This study aimed to determine the chemical content and antimicrobial activity of ethanol extracts of the combination of green betel leaves and Moringa leaves against Staphylococcus aureus, Escherichia coli, and Candida albicans. Green betel and Moringa leaves were macerated in 96% ethanol to extract secondary metabolites. Secondary metabolites were analyzed using phytochemical screening. Antimicrobial activity was tested using disk diffusion using various concentrations: EF1 (25% betel leaves + 75% moringa leaves), EF2 (50% betel leaves + 50% moringa leaves), and EF3 (75% betel leaves + 25% moringa leaves). Phytochemical tests showed that the ethanol extracts of betel leaves contained flavonoids, alkaloids, tannins, and saponins, and no terpenoids, while moringa leaves contain flavonoids, tannins, saponins, and terpenoids, but no alkaloids. S. aureus and E. coli had the highest inhibition zones at EF3 (75% betel leaves + 25% moringa leaves) concentrations, with average inhibition zones of 21.72 mm and 20.98 mm, respectively, and C. albicans at EF2 (50% betel leaves + 50% moringa leaves) concentration, with an average inhibition zone of 26.52 mm, with a very strong inhibition category.

The H5N1 bird flu virus continues to be endemic in Indonesia, posing continued threats to the poultry farming industry and public health. Classified as an influenza type A virus, the avian influenza virus is a member of the Orthomyxoviridae family. Type A influenza virus is the most lethal strain and frequently evolves resistance. The exploration of novel neuraminidase (NA) inhibitors is imperative due to the development of resistance by the H5N1 virus to NA drugs. Brucea javanica extract inhibits the activity of the H5N1 NA enzyme, according to previous research. Using the molecular docking technique, this study sought to ascertain the in-silico activity of the B. javanica compound in relation to H5N1 NA. By utilizing molecular docking simulation, we conducted the in-silico study and predicted the toxicity and pharmacokinetic profile of compounds, in addition to their drug-likeness according to Lipinski's Rule of Five. The result showed that Bruceantinol had a free binding energy of -8.93 kcal/mol, an inhibition constant of 0.28 M, and interactions with six important amino acid residues. The HIA and CaCo-2 values were 47.935% and 19.871%, respectively, whereas the PPB and BBB values were 39.591% and 0.049%. Neither is this substance carcinogenic nor mutagenic. Low binding energy and the most favored interaction with H5N1 NA were observed for Bruceantinol. Thus, Bruceantinol exhibits potential as a prospective NA inhibitor.

Radioisotope applications have become widespread in many fields, such as medical. For the optimal treatment using radioisotope, an accurate absorbed dose rate distribution calculation is required to provide the optimal dose for each patient. A software has been designed to calculate this absorbed dose rate distribution so that paramedics would know whether the absorbed dose rate distribution emitted from that particular source is sufficient or not. Using the TG-43 formula derived by American Association of Physicist in Medicine (AAPM), the calculation were performed using manufacturing data of the Ir-192 source and the function which involve the geometric position between the source and a calculation point. The results of this calculation which were viewed using the imageJ software, have shown that the absorbed dose rate for a source located in the center of  a volume was getting larger near the source location but has decreased drastically at the source location.This is due to the unavailability of manufacturing data for radius less than 2.5 mm. As for absorbed dose rate distribution emitted from several sources, the dose rate accumulated and reached the maximum in the middle. It has also formed some sort of shape depending on the location of the source, which form an isodose line or curves.Keywords: TG-43 Formula, absorbed dose rate distribution

The White Teak plant (G. arborea) and Sengon (F. moluccana) contain flavonoids that act as antioxidants and have the potential to lower blood sugar levels. This study aims to examine the effects of G. arborea and F. moluccana extracts with polar and non-polar (methanol and n-hexane) solvents on blood glucose levels as well as the histological condition of the pancreas and liver in mice. The extract was obtained through maceration, and its effectiveness was tested by measuring blood glucose levels and conducting histological analysis of the organs. Data were analyzed using SPSS. The research results show that extracts of G. arborea and F. moluccana lower blood glucose levels and improve the histology of the pancreas and liver in mice. Polar solvents (methanol) are more effective in lowering blood glucose levels, while non-polar solvents (n-hexane) have a greater impact on improving organ histology. In conclusion, the extracts of these two plants have a positive effect on the liver condition of hyperglycemic mice.

Pemanfaatan supernatan Bacillus subtilis kini telah banyak dimanfaatkan dalam bidang kesehatan sebagai probiotik karena mengandung senyawa bakteriosin serta enzim enzim. Senyawa tersebut mampu menghambat mikroorganisme lain dan mudah terdegradasi oleh enzim proteolitik. Pemanfaatan nanopartikel polimer cukup efektif untuk melindungi probiotik dari kondisi gastrointestinal. Penelitian ini bertujuan untuk membuat sediaan nanopartikel supernatan B. subtilis dengan pembawa PLGA dan dilakukan karakterisasi. Nanopartikel dibuat dengan metode solvent evaporation menggunakan variasi konsentrasi PLGA. Hasil karakterisasi nanopartikel supernatan B. subtilis memiliki ukuran nanopartikel 545,45 ± 90,73 nm dengan nilai indeks polidispersitas (PDI) kategori monodisperisi yaitu 0,503 ± 0,065 dan zeta potensial -14,57 ± 3,08 mV. Morfologi yang dihasilkan berbentuk sferis dan cukup seragam. Analisis Fourier transform infrared spectroscopy (FTIR) menunjukkan pita-pita spektrum mirip dengan nanopartikel PLGA dan menunjukkan beberapa puncak spesifik supernatan B. subtilis dengan sedikit pergeseran. Analisis Differential Scanning Calorimetry (DSC) pada termogram tidak menunjukkan adanya puncak endotermik dari supernatan B. subtilis. Formulasi nanopartikel supernatan B. subtilis telah ter-loading-kan ke dalam sistem nanopartikel melalui analisis FTIR dan DSC.

Dengue Haemorrhagic Fever is a disease caused by the dengue virus through a mosquito vector Aedesaegypti. NS3 Helicase is known as one of nonstructural proteins consisting of some essential enzymesfor virus replication. Nowadays ivermectin has been developed as an anti-dengue haemorrhagic feverwith therapy target NS3 Helicase. The therapeutics drug for dengue haemorrhagic fever has not beenfound specifically. Methanol extract of meniran (Phyllanthus niruri L.) reported the activity to denguevirus with a concentration of 15,63 μg/mL. This research aimed to study the interactions and affinityof the active compound of meniran with the receptor (NS3 Helicase) and to know ADME and toxicityprofile. From 56 active compounds of meniran, there was one best candidate as dengue haemorrhagicfever therapy which has energy binding (ΔG) and Inhibition Constanta (IC) lower than native ligandand ivermectin, it is nirurin with energy binding -4.87 kcal/mol. These candidate compounds havegood absorption and distribution profiles so they are thought to be candidates for dengue fever therapyby targeting the NS3 Helicase receptor which is better than ivermectin and native ligands.

Ibuprofen dikenal sebagai obat antiinflamasi nonsteroid yang digunakan untuk analgetik dan antipiretik dan termasuk obat dengan kelarutan rendah, tetapi memiliki permeabilitas yang tinggi. Penelitian ini bertujuan untuk menghasilkan ibuprofen dengan kelarutan dan disolusi yang lebih tinggi dengan membentuk mikropartikel menggunakan metode emulsification-ionic gelation. Partikel ibuprofen dikarakterisasi menggunakan Scanning Elektron Microscopy dan Fourier Transform Infrared Spectroscopy (FTIR). Kemudian dilakukan uji kelarutan dan uji disolusi ibuprofen. Pengujian karakteristik menggunakan SE, mikropartikel pH 2, pH4, pH 6 mengalami penurunan ukuran partikel berturut-turut sebesar 97,48%, 84%, dan 72% dari ukuran ibuprofen. Kelarutan ibuprofen setelah proses emulsification-ionic-gelation dalam air pada pH 6 meningkat delapan kali, sedangkan disolusi ibuprofen tertinggi ditunjukkan oleh partikel ibuprofen pada formula 3 yang dapat mendisolusi ibuprofen lebih dari 44% dalam 30 menit pada media dapar HCl 0,1N dibandingkan dengan formula yang lain. Kesimpulan yang diperoleh adalah partikel ibuprofen setelah proses emulsification-ionicgelation dapat memperkecil ukuran partikel dan meningkatkan kelarutan tetapi tidak dengan disolusinya.Kata kunci: Ibuprofen, emulsification-ionic-gelation, polivinil alkohol, kelarutan, disolusi

Bajakah root has stronger antioxidant activity than vitamin C. The Dayak people use it to maintain health. Bajakah root infusion has an unpleasant taste and aroma, also unstable, so effervescent tablets are developed to resolve that problem. Effervescent tablets are prepared by direct compression method. The starch of porang tuber is a food that can act as fillers and tablet binders. The co-processed excipient can produce tablet fillers for the directly compressed method. Co-processed excipients of lactose-porang starch have excellent flow properties, improve tablet hardness, have good compatibility, and are cheaper. In this study, the proportions of porang starch and lactose as co-processed excipients were determined using the Simplex Lattice Design method, and then tested the flow properties and granule repose angle. The physical properties of the tablets include weight uniformity, hardness, brittleness, dissolution time, and antioxidant activity test. Test data were analyzed using the One Sample T-test. The results showed that effervescent tablets with co-processed excipient porang starch-lactose 64: 36% provide good physical stability, granule flow time of 10.45 ± 0.29 gram/second, granule repose angle of 27.52 ± 0.95°; tablet weight uniformity with a CV value of 1.021%; tablet hardness 5.74 ± 0.07 Kg, tablet friability of 0.549 ± 0.03%; tablet disintegration time 86 ± 4 seconds. The bajakah root has antioxidant activity of 0.155 mg/mL.

Guava (Psidium guajava L.) has been widely used by Indonesian people as traditional medicine. Guava leaves have been studied and show various pharmacological activities including antidiarrheal, antioxidant, anti-inflammatory, analgesic, antimutagenic, anti-cough, antibacterial, antifungal, antiplaque, suppresses cardiac muscle inotropism, antidiabetic, antihypertensive, hepatoprotective and anticoagulant. In Indonesia, guava leaves have been formulated into several dosage forms. The guava leaves extract, as raw material of traditional medicine, should perform stability during storage period. This study aims to test the physical stability of guava leaves extract, which includes organoleptic, pH, and viscosity at different storage temperature and time susceptibility. Extraction was carried out by maceration method using ethanol 96% as solvent. Extraction is done until a thick extract is obtained. The extract was stored at 8oC (refrigerator temperature), 27oC (room temperature), and 70oC (oven temperature). Physical stability test was performed before storage period and at 1st, 2nd, 3rd week of storage period. Stability test results showed that ethanolic extract of guava leaves was stable at different storage temperatures. The results showed no differences on organoleptic, pH and viscosity test before storage period and at 1st, 2nd, 3rd week of storage period.  Key words: Physical stability, Guava leaves, Ethanolic extract

Illegal practices of formaldehyde as preservatives in wet noodles have been proven. These formaldehyde-preserved wet noodles are sold in West Java, Indonesia. This compound may be harmful to the body due to a decreasing of blood pressure, coma, acidosis, and acute renal failure. In this study we proposed a quantification method using high performance liquid chromatography (HPLC). A simulation was carried out as comparison. This simulation comprised of formaldehyde-spiked fish then the process was continued by washing, frying, and distilling the fish in a closed-system distillation. Similar condition was applied onto wet noodles purchased from traditional markets in Bandung. Method used was RP-HPLC derivatization method based on the reaction of formaldehyde carbonyl with two different reagents of DNPH (2,4-dinitrophenylhydrazine) and Nash (acetyl acetone, ammonium acetate and acetic acid) reagents. Result showed that formaldehyde residue was detected and quantified in all wet noodle samples with a range of 21-59 ppm. In conclusion, this method can be used for routine analysis to control illegal practices of formaldehyde in wet noodles. Moreover, washing and frying the formaldehyde-preserved food were proven could significantly help reducing formaldehyde level in food.Keywords: 2,4-dinitrophenylhidrazine, formaldehyde, RP-HPLC, wet noodles

Pain is a prevalent symptom of various diseases, characterized by subjective sensory and emotional experiences, often triggered by nerve irritation and pain receptor activation. Prostaglandins play a crucial role as chemical mediators in pain mechanisms. Clove oil-based liniment is commonly used for pain relief, with eugenol as its main active compound. This study investigates the antioxidant activity of VCO-clove liniment with different clove proportions using the DPPH method and evaluates the analgesic potential of eugenol through its interaction with the PTGS2 receptor through in-silico analysis. VCO-clove liniment was formulated with varying clove weight ratio (1:10, 5:10, and 10:10 w/v). Antioxidant activity was evaluated in-vitro using DPPH assay, while the interaction between eugenol and PTGS2 (PDB code: 5KIR) was predicted using molecular docking. The results demonstrated that the 10:10 ratio exhibited the highest antioxidant activity, with an IC50 value of 40.33 µg/mL and an antioxidant activity index (AAI) of 0.99, classified as moderate. In-silico analysis revealed strong binding between eugenol and PTGS2, with a binding energy of -5.22 kcal/mol, suggesting its analgesic potential. In conclusion, VCO-clove liniment shows promise as an antioxidant and analgesic agent, with the 10:10 ratio displaying optimal antioxidant properties, and eugenol contributing significantly to its analgesic effects through PTGS2 interaction.

Buah mengkudu mengandung sejumlah senyawa fitogenik. Lama penyimpanan yang berbeda dapat mempengaruhi kualitas produk. Selama penyimpanan berlangsung produk mikrokapsul ekstrak buah mengkudu dapat terjaga kualitasnya dan terlindungi senyawa bioaktifnya dengan metode mikroenkapsulasi. Tujuan penelitian adalah mengetahui pengaruh produk mikrokapsul ekstrak buah mengkudu dengan berbagai penyalut pada lama penyimpanan yang berbeda. Penelitian menggunakan penelitian experimental dengan Rancangan Acak Lengkap yang terdiri dari 10 perlakuan 3 ulangan. Setiap formulasi dilakukan penyimpanan selama 0, 2, 4 minggu. Peubah yang diamati adalah kadar air, aktivitas antioksidan dan sebaran jamur. Proses mikroenkapsulasi ekstrak buah mengkudu memberikan pengaruh yang nyata terhadap kestabilan kadar air, aktivitas antioksidan dan sebaran jamur dibandingkan dengan perlakuan yang tidak dilakukan proses mikroenkapsulasi. Produk mengalami peningkatan kadar air, aktivitas antioksidan dan sebaran jamur seiring dengan lamanya proses penyimpanan.

The occurrences of a highly pathogenic avian influenza virus (HPAI) type A H5N1 has caused infections in millions of poultry as well as hundreds of human cases and even mortalities. Indonesia has become one of the world’s highest casualty rates of H5N1 human infections, with the number of deaths was 167 from a total of 199 cases. The development of viral resistance towards the available anti-influenza drugs neuraminidase (NA) inhibitors required the discovery of new inhibitors. In the recent advance of drug discovery, natural products have been considered as one of the essential sources of medicinal agents, and Brucea javanica has been found to possess antiviral activity against H5N1 NA. Thus, this research aimed to investigate the in silico activities of compounds from B. javanica using molecular docking methods against H5N1 NA. In this study, docking-based virtual screening of compounds from B. javanica to quickly select in silico hits to be potential NA inhibitors was performed. Subsequently, the intermolecular interactions of the inhibitor compounds with the H5N1 NA were analysed to examine the most preferred interactions. The results showed that brucein G and bruceoside C were found having the lowest binding energy and most preferred interactions with H5N1 NA and therefore, can be proposed for further study as potential NA inhibitors.Keyword: antiviral, Brucea javanica, H5N1, molecular docking, neuraminidase

Sasar penelitian ini ialah untuk mengetahui dosis terbaik untuk histologi pankreas dan metabolits sekunders pada konsentrat etanolik daun pepaya (Carica papaya L). Penelitian ini merupakan percobaan laboratorium dengan 30 ekor tikus sebagai hewan uji. Hewan uji di bagi menjadi enam kelompok perlakuan masing-masing lima ekor tikus. Suspensi Na-CMC diberikan pada kelompok 2 (control negatif), glibenklamid diberikan pada kelompok 3 (control positif), dan 100, 200, dan 300mg/kgBB konsentrat etanol daun pepaya (Carica papaya L) diberikan pada kelompok 4, 5, dan 6. Konsentrat etanol daun pepaya mengandung senyawa metabolits sekunders antara lain alkaloids, flavonoids, saponins, dan tanins, sesuai temuan. Pepaya Etanol daun pepaya secara bermakna mempengaruhi gambaran histopatologi pankreas, Etanol daun pepaya terpisah pada porsi 300mg/kgBB merupakan bagian yang mempengaruhi gambaran histopatologi pankreas dengan skor tipikal 1,2.

Background: cinchona extract contains quinoline alkaloids, has telangiectatic activity, is practically insoluble in water so penetration into hair follicles is low and requires a delivery system, namely cream. Objective: to determine the formula of cinchona extract cream with good activity, physical stability and safety. Methodology: cinchona bark extraction (soxhletation method), phytochemical screening, cinchona extract dosage determination, optimization of cream formula using Design Expert software version 11. Evaluation: pH, adhesion, spreadability, viscosity and cycling test stability. Activity test using guinea pigs (Cavia porcellus) for 14 days, parameters of hair length/2 days, hair mass (14th day). Acute dermal irritation test using rabbit (Orycogalus cuniculus) by erythema and edema index parameters. Results: cinchona extract (13.32% yield) contained quinoline alkaloids, cinchona extract dosage was 20% (p<0.05). The optimum formula is F2 with characteristics: pH 6.48±0.07, adhesion 1.16±0.07, spreadability 4.99±0.85 and viscosity 3067±416.45, has good physical stability. Activity test: test area hair length 13.54 – 62.14% longer than normal control and 10.10 – 49.17% than negative control. The hair mass of test area was 122.91% heavier than normal control and 104.48% of negative control (p<0.05). Cinchona extract and excipients were non-irritant. Conclusion: Cinchona extract cream (20%) has hair fertilizer activity, good physical stability and safety.

Kalium dan kalsium merupakan makromineral, yang memiliki peran dan fungsi penting bagi tubuh, baik pada sel, jaringan, organ, dan keseluruhan tubuh. Kalium berfungsi untuk mengaktifkan enzim, serta membantu dalam menjaga tekanan osmotik dan keseimbangan antara asam dan basa, sedangkan kalsium berfungsi dalam pemeliharaan tulang dan gigi serta membantu kontraksi dan relaksasi pada otot. Salah satu sumber mikromineral yaitu ikan. Tujuan dari penelitian ini adalah untuk mengetahui kandungan kalium dan kalsium pada ikan kembung dan ikan gabus. Analisis kandungan kalium dan kalsium menggunakan metode Atomic Absorption Spectrophotometry (AAS), terdiri atas: preparasi sampel dengan metode destruksi basah (HNO3 35% dan H2O2 30%), validasi metode dan analisis sampel ikan kembung dan ikan gabus. Kadar kalium pada ikan kembung adalah 64.391,16 mg/100 g +97,62 dan pada ikan gabus adalah 30.988,70 mg/100 g +230,62 sedangkan kadar kalsium pada ikan kembung adalah 29.197,6607 mg/100 g +17,77 dan pada ikan gabus adalah 21.369,7065 mg/100 g +13,99. Kandungan kalium dan kalsium pada ikan kembung dan ikan gabus  sangat berbeda, hal ini dikarenakan habitat pada ikan kembung dan ikan gabus berbeda. Kata kunci: Ikan gabus, ikan kembung, kalium, kalsium, SSA